【摘要】 目的探讨宫颈癌组织端粒酶活性与人乳头状瘤病毒(HPV)感染的关系。方法采用原位PCR和原位杂交技术对66例宫颈癌和25例宫颈上皮内瘤变(CIN)组织进行检测。结果宫颈癌组织中端粒酶阳性率为92.4%(61/66),明显高于CIN的32.0%(χ2=33.24,P<0.001)。宫颈癌组织中HPVDNA阳性率为78.8%(52/66),52例HPVDNA阳性组织中51(98.1%)例端粒酶阳性(χ2=11.188,P<0.001)。结论端粒酶活性与HPV感染有密切关系。HPV感染在宫颈癌发生中可能有病因学意义,端粒酶活性增强可能是宫颈癌转化中的重要分子变化。更多还原

【Abstract】 Objective To study the relation between telomerase activity and human papillomavirus(HPV)infection in cervical cancer.Methods In situ hybridization and in situ PCR were used to detected the telomerase activity and HPV DNA in66cases of cervical cancer and in25cases of cervical intraepithial neoplasia (CIN).Results The rate of telomerases positive expression in cervical cancer was92.4%(61/66).It was significantly higher than that in the CIN (χ 2 =33.24,P<0.001).HPV positive rate was78.8%(52/66)in cervical cancer,of which telomerases of51(98.1%)cases is positive(χ 2 =11.188,P<0.001).Conclusion There is a close relationship of the HPV infection and telomerases activity.HPV infection might have etiologic significance for cervical cancer.The high telomerases activity could be a marker of biomolecular change in cervical cancer.更多还原