【摘要】 研究了典型光活化毒素α-三噻吩(简称α-T)对棉铃虫Helicoverpaarmigera和亚洲玉米螟Ostriniafurnacalis谷胱甘肽S-转移酶(GSTs)的影响。结果表明:近紫外光照(300～400nm)对棉铃虫和亚洲玉米螟幼虫GSTs活性无显著影响,但两种昆虫GSTs对α-T的反应不同。无光照条件下,α-T对亚洲玉米螟离体GSTs活性没有影响,而高浓度α-T能抑制棉铃虫离体GSTs活性;高剂量α-T可使两种昆虫活体GSTs活性升高。光照条件下,高浓度和高剂量α-T抑制棉铃虫GSTs活性,而不影响亚洲玉米螟离体GSTs活性,但低剂量α-T抑制其活体活性,而高剂量α-T则诱导其活性增加。棉铃虫和亚洲玉米螟GSTs对α-T反应的差异,可能与它们对药剂的敏感性以及药剂在两者体内的穿透、运转、贮藏、代谢等生理特性上的差异有关。更多还原

【Abstract】 The effects of αterthienyl (αT) on the glutathione Stransferases(GSTs) were investigated in Helicoverpa armigera and Ostrinia furnacalis. Results indicated that near ultraviolet irradiation (300~400 nm) had no effects on GSTs activities in the control larvae of H. armigera and O.furnacalis both in vitro and in vivo. Treatment with high concentration of αT inhibited the GSTs in vitro in H. armigera, but had no effects in O.furnacalis. Following topical administration, high doses of αT increased the GSTs activities in vivo both in two insects. Upon ultraviolet irradiation, high concentration of αT decreased the GSTs activities in vitro in H. armigera, But had no effects in O.furnacalis. After topical application, high dose of αT reduced the activity in vivo in H. armigera but induced it in O.furnacalis. The differential response of GSTs against αT in H. armigera and in O.furnacalis may be interpreted by their different sensitivities to αT. And the variances in penetration, transportation, storage and metabolism of αT may be also contributed to the differentiation.更多还原