【摘要】 从体内成熟与体外培养、激素处理或不处理以及在培养液中添加某些影响因子对哺乳动物卵母细胞核质成熟差异的现有研究资料进行了综述。更多还原

【Abstract】 Techniques of oocyte maturation in vitro affect the developmental competence of oocyte fertilization in vitro and embryo culture in vitro directly. It was reported that the requested environment or conditions were different between nuclear and cytoplasmic maturation in vitro. The criterion of nuclear maturation was indicated by the extrusion of first polar body and the oocyte reaching to metaphase Ⅱstage but there was no accepted standard for evaluating cytoplasmic maturation so far. Most of the researchers worldwide took the developmental competence of oocyte as their cytoplasmic maturation evaluating system. In general, cytoplasmic maturation of oocyte needs better conditions and longer culturing time than that of nuclear maturation in vitro. The conditions and results of oocyte maturation in vivo and in vitro, animals treated with hormones and some other factors affecting oocyte maturationin vitro and development in recent reports were discussed in the paper.更多还原