【摘要】 目的　建立一种简便、准确、灵敏、易于临床推广的测定血尿中儿茶酚胺 (CAs ,包括NE、E、DA)的检测方法 ,并进行了临床血浆及 2 4小时尿液标本中CAs测定。方法　采用活性氧化铝吸附标本中CAs,酸解吸附后 ,以 3,4-二羟基苄胺 (DHBA)为内标 ,利用离子对反相高效液相色谱及电化学检测器进行测定。结果　显示NE、E、DA在 0 .15 6 - 10 0ng/ml浓度范围内与响应值 (色谱峰面积对内标峰面积之比 )线性关系良好 (r分别为0 .9998、0 .9999、0 .9998) ,其方法回收率分别为 6 3.2 %- 93.4%、6 5 .6 %- 10 3.7%、70 .4%- 111.3%,CV值为1.2 3%- 19.5 2 %,在嗜铬细胞瘤患者中CAs有显著升高。结论　该实验方法简单、准确、灵敏、特异性高 ,不失为一种敏感、可靠的诊断嗜铬细胞瘤的方法。更多还原

【Abstract】 Objectives To establish a method to detect catecholamines in plasma and 24h urine by RP-HPLC-ECD. MethodsThe CAs in plasma and 24h urine were absorbed by Al 2O 3,then measured using DHBA as the interal standard.Results Linear calibration curves for NE,E,DA were measured within in a range of 0.156-100 ng/ml,and had a good correlation(r=0.9998- 0.9999),the recovery rate was 63.2%-111.3% and the intraassay precision(CV) was 1.23%-19.21% while interassay CV was 2.01-19.52%.Conclusions This was a simple,accurate,high specific method to detect CAs in the clinical samples and suggested to be a sensitive,reliable method for the dianosis of phechromocytoma.更多还原