【摘要】 在不同的培养温度下制备一系列大肠杆菌感受态细胞,并选取其中一组感受态细胞在4种不同的温度下保存,对培养温度与大肠杆菌感受态形成的关系以及感受细胞在不同保存温度下的保持情况进行了研究.结果表明,不同培养温度下大肠杆菌生长出现高峰期不同.其中,18℃和25℃,出现于对数期早期,而30℃和37℃则出现于对数期中后期.在25℃条件下转化效率最高.感受态细胞在4种不同温度条件下保存,受活菌数和感受态2种因素的影响程度都不同.转化效率在前期都有不同程度的提高,液氮条件下保存感受态细胞时间最长.更多还原

【Abstract】 A series of competent cells of Escherichia coli under different cultural temperatures was produced, and a givengroup of competent cells was chosen to keep under four different temperatures The relationship between efficiency of transformation and the cultural or preservation temperature was studied The result shows that different temperature results in different peak time At 18℃ and 25℃,peak time is at early time of log phase, while at 30℃ and 37℃, peak time is at middle and later time of log phase The highest transformation efficiency of competent appears at 25℃ Both the number of live cell and competent station affect the transformation efficiency, and the affecting degree is different at different temperatures At the early conservation time, transformation efficiency of all the temperature always increases And the temperature of liquid nitrogen contributes to the longest preservation time 更多还原