【摘要】 以绵羊β-乳球蛋白(β-lactoglobulin,BLG)基因5’端0.8kb片段,作为目的基因人肝再生增强因子(Humanaugmenterofliverregeneration,hALR)乳腺特异表达启动子,人巨细胞病毒(Humancytomegalovirus,CMV)启动子为绿色荧光蛋白基因(EnhancedGreenfluores-cenceprotein,EGFP)的启动子,构建了ALR基因乳腺特异表达而EGFP基因非组织特异性真核表达载体.这将为利用乳腺生物反应器生产人肝再生增强因子转基因动物及转基因早期胚胎鉴定奠定基础.更多还原

【Abstract】 hALR gene, linking a 0.8kb 5’end fragment of sheep BLG gene and EGFP gene with a CMV promoter were inserted in the proper orientation into pGEM7zf(+) in succession. As a result, a hALRspecific expression vector for sheep mammary gland has been constructed. This article provides a transforming vehicle for sheep mammary gland as bioreactor and a method of identifying the transgenic early embryo before gene transfer.更多还原