【摘要】 以甜瓜品种河套蜜瓜成熟果实mRNA为模板,经反转录合成和PCR扩增得到编码多聚半乳糖醛酸酶(Polygalacturonase,PG)基因全长cDNA,将其克隆于pUC19质粒中获得重组质粒pCMPG.此cDNA长1183bp,包括一个393个氨基酸残基组成的开放阅读框架.与已报道的甜瓜PG基因cDNA核苷酸序列比较同源性为99.3%,相应的氨基酸的同源性为98.5%.更多还原

【Abstract】 Total RNA was extracted from mature fruits of Hetao melons (Cucumis melo L.cv Hetao).The first strand of cDNA was synthesized and a fulllength cDNA encoding for polygalacturonase(PG) was obtained by RTPCR technique. This DNA was cloned into pUC19, then introducted into E.coli DH5α to obtain the recombinant pCMPG.The sequence of this cDNA was 1183bp in length,with an open reading frame of 393 amino acids . The cDNA nucleotide sequence and deduced amino acid sequence were highly homologous to that of the reported melon PG cDNA.更多还原