【摘要】 从蛋白质变性的构象渐变模型出发,分析了多结构域蛋白质变性过程的特点.用吸附动力学方法得到了可描述多个结构域变性的关系表示式,特别对典型双结构域蛋白质变性过程予以较详细的讨论.通过对比理论计算结果与实验观测结果,发现在一定参数条件下两者符合较好.从构象渐变的观点,对双结构域蛋白质变性过程中出现双S形变性曲线的原因做出了解释,同时对变性过程中可能出现的一些情况进行了预测和分析.更多还原

【Abstract】 The unfolding features of the multiple-domain proteins are analyzed in terms of sequential model.The formulae that can describe the protein unfolding processes of the multiple-domain proteins are deduced by means of adsorption kinetics.The unfolding processes of the typical two-domain proteins are discussed in details. Under certain conditions,the explanation far the double sigmoidal curves of two-domain proteins is given.At the same time, some expectations for several different unfolding processes are made.更多还原