【摘要】 目的 :探讨淋病奈瑟菌DNA回旋酶A亚单位GyrA基因及拓扑异构酶ⅣC亚单位ParC基因突变与耐喹诺酮类药物之间的关系。　方法 :采用DNA序列测定法对 2 0株无锡地区淋病奈瑟菌临床分离株的GyrA及ParC基因的喹诺酮耐药决定区 (QRDR)相关序列进行测序分析 ,并利用琼脂扩散法检测了该 2 0株淋病奈瑟菌临床分离株对诺氟沙星药物的敏感性。　结果 :2 0株被检淋病奈瑟菌菌株在GyrA基因的两个常见突变位点之一的Ser91处均未发生突变 ,却在另一位点Asp95处则均有突变 ;而 19株被检淋病奈瑟菌菌株中有 16株发生了ParC基因的 86、87、88、91位点突变。　结论 :GyrA基因的Asp95位点突变与ParC基因的Asp86、Ser87、Ser88和Glu91位点突变共同参与了淋病奈瑟菌对喹诺酮类药物产生的耐药。更多还原

【Abstract】 Objectives: To investigate the correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria Gonorrhoeae. Methods: The gene fragments of quinolone resistance-determining region (QRDR) in GyrA and ParC genes in 20 Neisseria gonorrhoeae strains clinically isolated in Wuxi, China, were sequenced, and the susceptibility of the 20 strains to quinolone was examined by agar diffusion method. Results: The mutations at the Asp95 point in GyrA gene were found in 20 strains. Of the 19 stains examined, 16 had mutations at the 86, 87, 88, 91 points in ParC genes. Conclusions: The mutations of Asp95 in GyrA gene and Asp86, Ser87, Ser88, Glu91 in ParC gene contribute to quinolone resistance in Neisseria Gonorrhoeae.更多还原