【摘要】 以甘蓝 (Brassiaca oleracea)为材料 ,取幼叶分离 m RNA,反转录合成 c DNA,以 c DNA第一链为模板 ,通过 PCR扩增 ,获得甘蓝磷酯酶 D (Phosphorate Lipid Dehydrolase,PLD) HKD2功能区的基因片段 .对其进行Blast分析 ,结果表明 ,分离的目的片段核苷酸序列与 Genbank中报道的甘蓝 PLD基因相比同源率为 99.7% ,只有 2个碱基发生改变 .将得到的 PLD基因片段插入植物表达载体 p AT940 ,构建了 PLD基因反义表达载体p ATC-r PLD,为下一步进行抗逆转基因作物选育打下基础 .更多还原

【Abstract】 mRNA were isolated from the young leaves of Brassica oleracea cultivar JinGan 8 and then cDNA were synthesized by reverse transcription. The HKD2 domain in PLD cDNA was amplified by PCR using cDNA first strand as templates. Sequence analysis indicated that the cloned cDNA fragment was showing 99.7% homology to the sequence reported before. Only two bases are different. The antisense expression vector with this fragment under the control of SP promoter were constructed and can be used on molecular breeding of salt-tolerant plants.更多还原