【摘要】 目的:观察板层蛋白（laminin,LN）在儿童系膜增生性肾小球肾炎（MsPGN）肾活检肾组织中表达的质或量的改变及其意义。方法:以板层蛋白α₁、α₂、β₁、β₂、γ₁结构链为观察对象,用免疫组化和原位杂交技术观察它们在18例系膜增生性肾小球肾炎肾活检组织和6例正常成熟肾组织中的表达和分布。结果:①LNα₁;、LNγ₁在肾小球系膜增殖节段蛋白表达增强;LNα₂、LNβ₁在部分增殖的肾小球系膜区出现节段性异常表达;LNβ₂链在增生的系膜区和肾小球基膜（GBM）表达出现不同程度的下降。②LNα₁、α₂、β₁、和γ₁的mRNA在系膜增生的肾小球内表达有不同程度的增加,但轻度、中度和重度系膜增生的组间无明显差异。结论:①在系膜增生性肾小球肾炎中板层蛋白的表达既有量的改变也有质的变化,增生的系膜细胞是肾小球内板层蛋白量的积聚和异常合成的重要细胞来源。②板层蛋白各分子链分布的改变可能是肾小球功能异常的病理基础。更多还原

【Abstract】 Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization. Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of prolifera-tive mesangium. Increased expression of α2 and β1 proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of α1, α2, β1 and γ1 increased to different degrees in glomeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:① The quantitative and qualitative alterations of laminin chains’ distribution were found in the measngial proliferative glomeruli. The proliferative mesangial cells were the origins of abnormal accumulation and expression of laminins. ② These changes may be the basis of the progresses of MsPGN.更多还原