【摘要】 目的:研究转基因中国仓鼠卵（CHO）细胞中单胺囊泡转运体（VMAT₂）的抗毒性作用。方法:利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞（cDNA-CHO）,采用MTT比色法检测1-甲基-4-苯基吡啶离子（MPP⁺）对CHO细胞野生株（wtCHO）和cDNA-CHO细胞的毒性作用,并观察利血平——VMAT₂的特异性阻滞剂对MPP⁺毒性作用的影响。结果:在MPP⁺ 0.5mmol/L以上浓度cDNA-CHO细胞对MPP⁺敏感性比wtCHO低得多;cDNA-CHO和wtCHO对鱼藤酮（rotenon）的敏感性无显著差异;加入利血平后,上述保护作用消失,cD-NA-CHO对MPP⁺敏感性与wtCHO细胞无差异,而单独予以wtCHO细胞利血平则不能改变它对低浓度MPP⁺的敏感性。结论:此保护机制是由转基因细胞中VMAT₂引起的,VMAT₂在转基因的非神经细胞系（CHO细胞系）中也能将MPP⁺转运至囊泡内,从而保护细胞,同时也提示PC12细胞内具有抗毒性作用的成分。更多还原

【Abstract】 Objective : To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transgenic Chinese Hamster ovary (CHO) cell. Methods :With the technology of trans-gene from PC 12 to CHO, MTT reduction assay was used to detect MPP+ toxic effect on wild type CHO (wtCHO) and transgenic CHO. Meanwhile, the role of reserpine was also observed in MPP+ toxic effects. Results :The sensitivity of transgenic CHO to MPP+ was much less than that of wtCHO with 0. 5 mmol/L MPP+. Transgenic CHO had the same sensitivity as wtCHO if rotenone was given. WtCHO, by given reserpine alone, didn’t change its sensitivity to MPP+. Conclusions :VMAT2 has protective effect on transgenic CHO by transporting MPP+ to vesicles.更多还原