【摘要】 目的:探讨载脂蛋白E（apoE）基因多态性与早发冠心病患者的关系以及对中国人血脂水平的影响。方法:选择68例年龄≤55岁的冠心病患者（CHD₁）、136例年龄≥65岁的冠心病患者（CHD₂）及136例健康对照患者,测定空腹血清甘油三酯（TG）、总胆固醇（TC）和高密度脂蛋白胆固醇（HDL-C）水平;应用PCR,HhaI内切酶消化及PAGE的方法确定apoE基因 多态性。结果:apoE3/4基因型频率和E₄等位基因频率在CHD₁组明显高于CHD₂组和健康对照组,CHD₂组和健康对照组无差别。CHD₁与对照组血脂水平无差别,而CHD₂组的TC、低密度脂蛋白胆固醇（LDL-C）明显高于CHD₁组和健康组。不同的apoE基因型TC、LDL-C的浓度存在显著性差异,按E₂（E2/2+E2/3）<E3（E3/3）<E4（E4/4+E3/4）递增。结论:apoE多态性是影响TC、LDL-C水平的重要遗传因素之一;apoE₄与CHD有明显的相关性,即apoE₄可能是早发冠心病独立的易患遗传危险因素。更多还原

【Abstract】 Objective :To assess the relationship between apolipoprotein E(apoE) polymorphism and early onset of Coronary heart disease(CHD) and the effect of apoE on lipids and tipoproteins in Chinese healthy subjects. Methods:Sixty-eight cases (CHDl) aged less than 55 y, 136 coses (CHD2) aged more than 65y with CHD and 136 healthy subjects were enrolled and their -plasma levels of triglyceride(TG) , total cholesterol (TC) and high density Upoprotein cholesterol (HDL-C) were determined. The apoE genotypes were identified by polymerase chain reaction-restriction fragment lengths polymorphism. Results -apoE3/4 genotype and E4 allele frequency in CHDl were higher than those in CHD2 and healthy subjects and no difference was found between CHD2 and healthy subjects. Meanwhile the plasma levels of TC and low density Upoprotein cholesterol(LDL-C) were higher in CHD2 than in either CHDl or healthy subjects. Each apoE isoprotein has variable TC and LDL-C levels characterized by E2 (E2/2 + E2/3) <E3(E3/3) <E4(E4/4 + E3/4). Conclusion.apoE is one of the genetic factors that affect the TC and LDL-C levels and apoE4 may be an independent genetic factor of early onset of CHD.更多还原