【摘要】 目的:探讨人胚胎肾脏成纤维细胞培养方法。方法:利用人胚胎肾脏肾髓质进行培养,然后通过细胞形态学、超微结 构、免疫细胞化学等方法进行分析鉴定。结果:培养的细胞为长梭形、单核,超微结构为典型的成纤维细胞,并表达成纤维细胞 表面抗原、波形蛋白、结蛋白,不表达角蛋白。结论:成功地培养出人胚胎肾间质成纤维细胞。更多还原

【Abstract】 Objective: To explore the method to culture human fetal renal interstitial fibrqblasts in vitro. Methods: The renal interstitial fibroblasts from human fetal kidney grew up in culture median. They were identified by morphology, ultrastructure examination and immunocytochemistry. Results: (1) The culture cells were fusiform mononuclear mono-nuclei cells with typical ultrastructure of fibroblast under transmission electron microscope. (2) The cells expressed vimentin, desmin and fibroblast surface antigen positively, but no expression of keratin. Conclusion: The method was successfully utilized to culture highly pure human fetal renal interstitial fibroblasts.更多还原