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亚铁对杂交水稻幼苗生长和部分生理生化特性的影响

Effects of EDTA-Fe2+ on Growth and Physio-chemical Characteristic of Hybrid Rice Seedling

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【作者】 王贵民陈国祥杨艳华刘少华邵志广

【Author】 Wang Guimin1,Chen Guoxiang1,Yang Yanhua1,Liu Shaohua1,Shao Zhiguang2(1.School of Life Science,Nanjing Normal University,210097,Nanjing,PRC)(2.Jiangsu Province Danyang Teacher’s School,212300,Zhenjiang,PRC)

【机构】 南京师范大学生命科学学院江苏省丹阳师范学校 210097南京210097南京212300丹阳

【摘要】 通过溶液培养研究不同浓度的螯合态亚铁(EDTA Fe2+)胁迫对水稻幼苗的生长、光合速率、丙二醛和超氧阴离子的含量及抗氧化酶活性的影响.结果表明:高浓度螯合态亚铁(EDTA Fe2+)胁迫明显抑制水稻地上部分和根系的生长,水稻叶片MDA和O 2的含量增高、光合速率下降.CAT,SOD在低浓度的条件下活性升高,高浓度条件下活性下降,在水稻抗铁毒害中起到一定保护作用;而POD在致死浓度条件下仍具较高的活性,在水稻抗铁毒害中起到主要保护作用.

【Abstract】 A solution culture experiment was conducted to study the effects of different concentrations of EDTAFe2+on the growth,photosynthetic rate,concentrion of MDA O2,activities of SOD?CAT and POD in the hybrid rice seedling leaves. The results showed that higher EDTAFe2+concentrations obviously inhibited the growth of shoot and root;And the contents of MDA and O2 were higher than the control. Under lower EDTAFe2+concentrations stress,the leaf photosynthetic rate and the activities of protective enzymes were increased under higher EDTAFe2+stress,however,the photosynthetic rate decreased. Higher EDTAFe2+stresses affected the activity of SOD and CAT more than POD. Under lethal EDTAFe2+concentration,The POD kept higher activity.It indicated that POD acted a mostly important role in the detoxification of EDTAFe2+ stress.

【关键词】 杂交水稻螯合态铁溶液培养
【Key words】 hybrid ricechelated ferrous ironsolution culture
【基金】 江苏省高校自然科学研究计划项目(02KJB180006);江苏省高校资源生物技术重点实验室开放课题(KJS01036).
  • 【文献出处】 南京师大学报(自然科学版) ,Journal of Nanjing Normal University(Natural Science Edition) , 编辑部邮箱 ,2003年02期
  • 【分类号】S511
  • 【被引频次】42
  • 【下载频次】331
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