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用微生物对六六六污染土壤进行生物修复的初步试验研究
Primary Study on Biomediation of Contaminated Soil with Hexachlorocyclohexane (BHC) by Microorganisms
【摘要】 采用田间采集土样、室内培养的方法,进行了在含有一定浓度六六六的土壤中添加特别筛选的BⅠ细菌进行降解等初步试验。研究结果表明,蓖麻籽粕对BⅠ细菌数量扩增有较好的促进作用;30d是试验条件下外加BⅠ细菌生长的最旺盛期,BⅠ细菌对六六六降解效果最好的时期可能是试验进行后的30d左右;试验进行的30d内,六六六浓度1~6mg·kg-1范围内的土壤,对BⅠ细菌的数量扩增没有太大的抑制作用,六六六浓度达到和大于18mg·kg-1时,对BⅠ细菌产生了非常显著的毒害作用,12~18mg·kg-1是本试验条件下BⅠ数量扩增的临界范围值;土壤中加1~6mg·kg-1六六六,并没有对外加BⅠ菌液的土壤过氧化氢酶活性产生明显的抑制,较低含量范围内的六六六似乎有刺激添加BⅠ菌液土壤中的过氧化氢酶活性的作用;初步试验的结果表明加入BⅠ细菌对土壤中污染物六六六的降解有较明显的效果。
【Abstract】 The primary experiment of adding BⅠ bacteria to a soil polluted by BHC, a very difficultly degraded organic hydrocarbon insecticide, indicated that castor bean- cake was the best material for reproduction of BⅠ bacteria among a few substances used in the experiment. The number of BⅠ bacteria added in soil increased to a vivid period after 30 days, when possibly was a very good period for BHC degradation after BⅠ bacteria added to the soil. On the other hand, it has been indicated that BⅠ bacteria were not inhibited remarkably during the first 30 days at concentration of 1 ~ 6 mg· kg- 1 for BHC added to the soil. But the bacteria exhibited a remarkable inhibition while the BHC′ content was higher than 18 mg· kg- 1. It may presumed that BHC’ content of 12~ 18 mg· kg- 1 was a critical value for BⅠ bacteria reproduction. No effect on catalase activity was found in the soil receiving BⅠ bacteria while BHC’ content was among 1~ 6 mg· kg- 1, and even BHC with low content seemed to excite some catalase activity. It is suggested that BⅠ bacteria degrade BHC remained in soil effectively.
【Key words】 bioremediation; hexachlorocyclohexane (BHC); microorganism; degradation;
- 【文献出处】 农业环境科学学报 ,Journal of Agro-environmental Science , 编辑部邮箱 ,2003年06期
- 【分类号】X172
- 【被引频次】22
- 【下载频次】390