【摘要】 目的　建立检测TCRDβ JβsjTRECs的方法 ,并了解不同T细胞受体Dβ Jβ之间T细胞受体删除DNA环(sjTRECs)在胸腺细胞和外周血T细胞中的形成情况。方法　利用巢式PCR分别扩增 10例正常人外周血单个核细胞和 3例正常胸腺细胞DNA中不同的Dβ片段和Jβ重排时形成的sjTRECs的情况 ,PCR产物进一步进行克隆和序列分析以确定结合区的位置。结果　可检测到Dβ1与 5个Jβ1基因片段、Dβ2与 4个Jβ2基因片段分别形成的sjTRECs,其中以Dβ1 Jβ1S1、Dβ1 Jβ1S2和Dβ2 Jβ2S2的sjTRECs最常见 ,PCR产物经克隆和序列分析证实其形成Dβ JβsjTRECs的情况。 结论　成功地建立了检测Dβ Jβ sjTRECs的方法 ,为分析各TCRβ亚家族的sjTRECs含量和确定TCRβna ve细胞提供了新方法更多还原

【Abstract】 Objective To establish the method of Dβ-Jβ sjTRECs detection for analysis the generation of TCR Dβ-Jβ signal joint T-cell receptor excision DNA circles (sjTRECs) within thymocytes and peripheral blood T cells in normal individuals.Methods The different Dβ-Jβ sjTRECs were amplified in genomic DNA from 10 cases of peripheral blood mononuclear cells and 3 cases of thymocytes, by using nested PCR. The PCR products were further cloned and sequenced to define the position of the joints.Results The sjTRECs of Dβ1 with 5 Jβ1 segments and Dβ2 with 4 Jβ2 segments were detected, predominantly in Dβ1-Jβ1S1, Dβ1-Jβ1S2 and Dβ2-Jβ2S2 sjTRECs. The sequences of Dβ-Jβ sjTRECs generation form some PCR products were identified by cloning and sequencing.Conclusions In the present study, the method of detection of Dβ-Jβ sjTRECs was successfully established, it may provide a new technique for analysis of TCRβ subfamily sjTRECs and identify TCRβ nave T cells.更多还原