【摘要】 目的　探讨家蚕蛹表达的重组乙型肝炎表面抗原中蛋白 [rHBsAg(PreS2 +S) ]口服免疫原性。方法　选择 4周龄的SD大鼠 ,将重组病毒感染后的家蚕蛹 ,制成口服剂型灌胃大鼠 ,ELISA方法检测抗 HBs抗体及抗 PreS2抗体。结果　在灌胃重组蛋白组中有 4 0 %鼠体血清内检测到了特异性抗 HBs抗体及抗 PreS2抗体 ,抗体应答反应持续 2～ 3周 ;口服免疫后抗体转阳的SD鼠加强免疫后能产生较强的二次免疫应答。进一步研究表明 ,口服家蚕蛹表达的rHBsAg(PreS2 +S)具有加强免疫的效果。对酵母重组疫苗单剂腹腔注射过的SD鼠 ,用家蚕蛹表达的rHBsAg(PreS2 +S)灌胃加强免疫后 ,能诱导鼠体迅速产生较强的抗 HBs抗体。结论　家蚕 BmNPV表达载体系统将有望开发成为新型的口服乙型肝炎疫苗表达系统更多还原

【Abstract】 Objective To determine the oral immunogenicity of silkworm pupa-derived rHBsAg (PreS2+S).Methods Gavaging with silkworm pupae producing rHBsAg (PreS2+S) to rats at 4 weeks of age and detecting anti-HBs and anti-PreS2 antibodies with ELISA. Results Anti-HBs and anti-PreS2 antibodies were detected in 40 percent rats gavaged. A stronger secondary antibody response was obtained only in the rats of antibody conversion after the booster injection. We also found that rHBsAg(Pre+S)-silkworm pupae gavaged to rats could be used to boost an immuneresponse. Rats primed with a single yeast-derived rHBsAg and gavaged silkworm pupae containing rHBsAg(Pre+S) developed immediate antibody responses.Conclusion Silkworm-BmNPV expression vector system will widen the scope, as novel expression system to develop edible HB vaccine.更多还原