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高温胁迫对Bt棉叶片杀虫蛋白表达量和氮代谢影响的研究

The Effects of the High Temperature Stress on the Leaf Bt Protein Content and Nitrogen Metabolism of Bt Cotton

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【作者】 陈德华杨长琴陈源聂安全吴云康

【Author】 CHEN Dehua1,YANG Changqin1,CHEN Yuan1 , NIE Anquan2, WU Yunkang1 nce, Yangzhou University, Yangzhou 225009, China; 2.Shanxi Cotton Academic Institute, Yuncheng 044000, China)

【机构】 扬州大学农业部江苏省作物栽培生理重点实验室山西棉花研究所扬州大学农业部江苏省作物栽培生理重点实验室 扬州225009扬州225009运城044000扬州225009

【摘要】 通过对两个不同类型Bt棉品种(系)不同生育期高温胁迫及持续时间研究表明,高温胁迫(37℃)48h对盛蕾期和盛花期叶片Bt杀虫蛋白含量影响不大。但在结铃盛期,高温使Bt杀虫蛋白含量明显下降。48h的持续高温结果进一步表明,科棉1号36h前下降快,新洋822于36h后下降快。高温使蛋白酶和肽酶活性提高,丙酮酸转氨酶活性下降,但氨基酸含量增加,说明可溶性蛋白分解增强。

【Abstract】 The experiment of high temperature on the two different types of Bt cotton cultivars was conducted at different developing periods, the results showed that the high temperature at 37℃ for 48 hours had less effect on the leaf Bt protein content in the peak squaring period and the blooming period; however, the Bt protein content decreased significantly in the peak boll period, and that of Kemian No.1 decreased significantly after being treated within 36 hours, while that of Xinyang 822 dropped sharply after 36 hours. Further more, the key enzyme activity of the nitrogen metabolism showed that the leaf protease and peptidase activity increased, the leaf GPT activity decreased but the content of amino acid increased during high temperature treatment of 37℃ for 48 hours at peak boll period. 

【关键词】 Bt棉高温胁迫Bt蛋白氮代谢
【Key words】 Bt cottonhigh temperature stressBt proteinnitrogen metabolism
【基金】 江苏省开放实验室资金(K2071);江苏省科技厅推广资金(BC2001330);江苏省三项工程资金(S01 58)
  • 【文献出处】 棉花学报 ,Acta Gossypii Sinica , 编辑部邮箱 ,2003年05期
  • 【分类号】S562
  • 【被引频次】59
  • 【下载频次】290
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