【摘要】 用经过改进的胍盐法提取了陆地棉品种珂字201胚性愈伤组织的总RNA,进而分离了mR-NA,合成了全长cDNA,并将其克隆到λTripIEx载体中,进行体外包装后,成功地获得了cDNA文库。该文库的滴度为2.02×107pfu·ml~1,重组效率在90％以上,插入片段大小范围也符合cDNA文库构建的要求。更多还原

【Abstract】 The embryogenic callus total RNA was extracted by a modified guanidinium salt method, then mRNA was isolated using Mcs-sageMaker reagent from upland cotton cv. Coker 201. Full-length cDNA was synthesized and cloned into phage λTriplEx 2 vector employing Clontech kit. After being packaged in vitro, the tiler of the library was measured as 2. 02 × 107 pfu·ml1 ,and the recombination rate was above 90%. The insert fragments also met the requirement of a good cDNA library.更多还原