【摘要】 用对Bt毒蛋白(Cry1Ac)具有一定抗性水平的安阳品系和相对敏感的新疆品系棉铃虫进行Bt毒蛋白(Cry1Ac)的室内毒力测定结果表明,两品系的LC50值分别为8.7645g·L 1和0.2547g·L 1,前者是后者的34.4倍。用转Bt基因抗虫棉中棉所29和常规棉花品种中棉所35蕾期的倒3叶饲养安阳品系和新疆品系棉铃虫的初孵幼虫(孵化后24h),结果表明:两个棉铃虫品系在取食抗虫棉后的累计校正死亡率在1～2d内差异不明显;新疆品系棉铃虫在转Bt基因抗虫棉上发育的最高龄期为4龄,其比率为6.7%;而安阳品系部分棉铃虫能够在转Bt基因抗虫棉上完成生长发育,其4龄幼虫的比率为45.5%,5、6龄幼虫的比率分别为12.6%和3.8%,有1.6%的幼虫能够正常化蛹;用常规棉棉叶饲养的安阳品系和新疆品系棉铃虫各龄幼虫的发育历期差异不显著,而用转Bt基因抗虫棉棉叶饲养的安阳品系棉铃虫幼虫的发育历期与新疆品系虽然差异不显著,但前者的发育历期均短于后者。更多还原

【Abstract】 Toxicity test shows that the LC50 values of Anyang and Xinjiang strains are 8.7645 and 0.2547 g·L1,respectively.The former is 34.4 fold of the later.Using the third leaves from the top of transgenic Bt cotton CCRI29 and nontransgenic Bt cotton CCRI35 feed the neonates of the two strains.The results are: the two strains’ accumulated mortality have not significant difference within 1～2d, but from the second to 23rd,the accumulated deathrate are parellel lines, showing the Anyang strain is resistante to transgenic Bt cotton; The Xinjiang strain’s highest larvae instar is the fourth instar on transgenic Bt cotton and the ratio is 6.7 %, but Anyang strain could complete development on transgenic Bt cotton. The ratio of fourth instar is 45.5 %,the ratio of fifth,sixth and pupae are 12.6%?3.8% and 1.6%, respectively; The developing durations of the two strains have no singnificant differrnce on transgenic and nontransgenic Bt cotton ,but Anyang strain has a shorter developing durations than that of Xinjiang strain.更多还原