【摘要】 通过对菌株同类型的nit突变体互补测定方法,对棉花红腐病菌(串珠镰孢霉)氮代谢途径相关因子的基因位点进行研究。根据对单孢菌株SC50的42个含钼协同因子缺陷型突变体(nitB)的互补测定,鉴定出至少有7个基因位点控制含钼协同因子;对硝酸盐还原酶缺陷型突变体(ni tA)间以及亚硝酸盐还原酶缺陷型突变体(nitC)间互补测定的结果表明,这两种酶均由多个基因位点控制,并有其它基因参与互作。更多还原

【Abstract】 Studies on gene loci of molybdenum containing cofactor and other enzymes related to nitrogen metabolic pathway in "SC50" strain, isolate of Fusarium moniliforme, were carried out by physiological complement test with nit mutants. Complement analysis of forty two molybdenum containing cofactor mutants (nitB) revealed that at least seven different genes were involved. Likewise, the results of complement tests for all nitrate reductase mutants (nitA) and all nitrite reductase mutants (nitC) also revealed that there were more than one gene to control and regulate the enzymes both,nitrate reductase and nitrite reductase.更多还原