【摘要】 利用石蜡制片 ,观察了西番莲双受精的全过程 ,其主要结果如下 :人工授粉后 6.5小时 ,大量花粉管进入子房腔 ,并沿子房内壁生长 ,进行珠孔受精 ;7～ 7.5小时 ,花粉管由珠孔进入胚囊 ,破坏一个助细胞 ,释放出二个精子 ;7.5～ 9小时 ,大多数胚珠雌、雄性核发生融合。其受精作用属于有丝分裂前型的配子融合类型 ;精子与卵细胞、精子与极核融合几乎同时发生 ,两极核于受精后融合 ;合子与初生胚乳核无休眠期 ,受精后立即进行有丝分裂。更多还原

【Abstract】 The double fertilization process of Passiflora edulis f.flavicarpa Deg. was observed via paraffin sections staining with Haematoxylin and feulgen reaction, and the results are as follows:After pollination, pollen grains germinated soon on the stigma within 30 minutes, and then the pollen tubes grew down the transmitting tissues of the styles. After 6.5 hours,the pollen tubes reached the ovary and grew along inner wall of the ovary. After 7～7.5 hours, the pollen tubes ran through the nucellar beak to the embryo sac and relesed the contents with two sperms.In the most ovules after 7.5～9 hours of pollination,the sperms were attached to the egg cell plasmas membrane,then entered into the nucleus.The sperm chromatin showed dispersion and the male nucleolus appeared.The male and female nucleoli were combined and fused to form a single nucleolus,and the egg cell came to the fertilized.Fertiliztion of polar nucleus is in the same time and similar to the egg cell fertilization.The fertilization of P. edulis f. flavicarpa Deg. is of premitosis type. Dormant period of fertilized egg cells and primary endosperm nucleus was not observed.Cell division take place immediately after fertilization finished.更多还原