【摘要】 就滇中一带云南松林进行了多次调查 ,共采集、鉴定标本 1 30余号 ;从中选取 1 3科 ,1 9属 ,33种真菌进行了菌种分离、培养的研究。选用并修改 4种培养基进行了外生菌根真菌分离、培养的比较 ,结果共有 1 1科 ,1 7属 ,2 5种真菌分离获得成功 ,成功率高达 75 .8% ,4种培养基的分离成功率依次为 36.4%、63.6%、33.3%和 5 7.6%。其中 ,修改PDA培养基和松针、玉米培养基较适宜于云南松外生菌根真菌的分离、纯化和培养。更多还原

【Abstract】 Several surveys were carried out under Pinus yunnanensis Franch forest in Central Yunnan; the total collections are over 130. 33 species, belonging to 19 genera, 13 families were selected to supply a diversity of pure cultures. 4 types of media were selected for the pure culture study of ectomycorrhizal fungi. As a result, pure cultures of 25 species, 17 genera, and 11 families were obtained. The total isolation success rate reached 75.8%; the success rate of 4 media (No.1, No2, No.3, No.4) was 36.4%, 63.6%, 33.3% and 57.6% respectively. Modified PDA medium (No.2) and pine needles medium (No.4) are more suitable for the isolation, purification and maintenance of ectomycorrhizal fungi associated with P. yunnanensis.更多还原