【摘要】 对花生品种鲁花10号原生质体分离与培养条件进行研究。以鲁花10号的体细胞胚作为供体,用含有2.0%CellulaseonozukaRS和0.1%PectolyaseY-23的酶溶液分离获得大量原生质体。将其培养在添加1mg/LNAA和4mg/LBAP的改良MS液体培养基中。培养约2～3d后,细胞开始分裂。然后部分细胞继续分裂,并形成细胞团。培养7～8周后,将形成的直径为1～2mm的小愈伤组织转移到添加1mg/LNAA和5mg/LBAP的MS固体培养基上培养,小愈伤组织迅速生长。3～4周后,愈伤组织长至7～9mm。更多还原

【Abstract】 The protoplast isolation and culture conditions of peanut (Arachis hypogaea L.) were studied. Protoplasts from somatic embryos of Luhua No. 10 were isolated with the enzyme solution containing 2.0% Cellulase onozuka RS and 0.1% Pectolyase Y-23, and then cultured in the modified liquid MS medium containing 1mg/L NAA and 4mg/L BAP. The first cell division occurred within 2～3 days. Then some of the cells divided and developed into colonies. After 7 to 8 weeks of incubation, protoplast-derived calli up to 1～2 mm in diameter were transferred onto the solid MS medium supplemented with 1 mg/L NAA and 5 mg/L BAP, for callus proliferation. After 3 to 4 weeks of transferring, the calli grew to 7～9 mm in diameter.更多还原