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辐射百合雄性不育突变体的RAPD分析

RAPD Analysis on Male Sterility Mutant Initiated from Irradiation Lily

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【作者】 张克中赵祥云陆长旬黄善武张启翔

【Author】 ZHANG Kezhong1, ZHAO Xiangyun1, LU Changxun2, HUANG Shanwu2, ZHANG Qixiang3(1 Department of Landscape and Gardening, Beijing Agricultural College, Beijing102206,China;2 Institute of Vegetable and Flowers, Chinese Academy of Agricultural Science, Beijing100081, China;3College of Landscape and Gardening, Beijing Forestry University, Beijing100083, China)

【机构】 北京农学院园林系中国农业科学院蔬菜花卉研究所北京林业大学园林学院 北京 102206北京 102206北京 100081北京 100083

【Abstract】 RAPD analysis on Lilium regale and its 13 phenotype male sterility mutants initiated from irradiation bulbs was made by means of 300 random 10mer primers. Of the tested primers, 93 primers produced ideal amplification bands on all materials, 14 primers generated stable different polymorphic bands among mutant ’Wang 2G03’?mutant ’Wang 3G03’ and Lilium regale. 21 different polymorphic bands were found between ’Wang 2G03’ and Lilium regale. 25 different polymorphic bands existed between ’Wang 1G03’ and Lilium regale. It was showed that ’Wang 2G03’ and ’Wang 1G03’were two real different male sterility mutants of Lilium regale. From the 93 primers RAPD bands, the other 11 phenotype male sterility mutants showed no difference in comparison with Lilium regale. It was proposed that more test need to be made to identify whether they were real mutants.

【基金】 北京市自然科学基金项目(6982008)的部分研究内容
  • 【文献出处】 林业科学研究 ,Forest Research , 编辑部邮箱 ,2003年03期
  • 【分类号】S682.29
  • 【被引频次】65
  • 【下载频次】300
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