【摘要】 对28年生的撑麻7号竹的离体快速繁殖技术进行了系统研究,结果发现:降低培养基中大量元素用量,提高BA质量浓度有利于形成丛芽;在按正交设计的9组增殖培养基中,芽的增殖率差异不显著,芽的生长量相差近4倍,而且凡配方中加有KT的苗的生长均比同质量浓度BA而未加KT的苗要好,表明KT有促进生长的作用;经反复试验比较,认为3 4MS(m)+BA2mg·L-1+KT1 25mg·L-1较适合增殖培养;还发现试管苗在萌发新芽时最易生根,采取先将苗生根培养20～30d后,转入增殖培养5～7d,可以使苗同步地萌发新芽,此时再将苗转入生根培养,采取2次生根的办法使生根率达到78 2%,从而解决了试管苗生根时大量死亡的问题。更多还原

【Abstract】 Systematic research was taken on in vitro rapid propagation of the hybrid of 28yearold (Bambusa pervariabilis×Dendrocalamus latiflorus)No.7 The results are as follows:(1) Both reducing the salt concentration of MS medium and raising BA(6Benzyl aminopurine) consistency favor the forming of clumping shoots; (2) In 9 sets of mediums for shoot proliferation, which were designed according to the diagonal intersection, the difference of shoot increase rate is not notable, the growth capacity of shoots differs near 4 times and in all the formula, if with the same BA concentration, the growth condition of the plantlets is better with KT(Kinetin) than that without KT in medium, which indicates that KT has an improving function on shoot growth; (3) The medium of 3/4 MS+BA 2 mg·L-1+KT 125 mg·L-1 is fit to shoot proliferation; (4) New shoots are easy to root, culturing shoots in rooting medium for 20～30 days and then in proliferating medium for 5～7 days can make new shoots come up synchronously, the new shoots are cultured in rooting medium again and the rooting rate can achieve 782%. In this way the problem of shoot dying in rooting medium is solved. Following the experience mentioned above, many species such as Dendrocalmus minor var. amoen and Dendroclamus asper ect.(all are more than 30 years old) have been succefully cultured.更多还原