【摘要】 目的　调查蒙古、藏、维吾尔、壮、彝、傣族 6个少数民族人群 CCR5 -Δ3 2等位基因的突变率和 CCR5基因在细胞表面的表达 ,及其在汉族人群中的分布状况。方法　每个民族抽取 1 0份标本 ,采用全血基因组 DNA提取方法提取DNA,运用 PCR法检测 CCR5 -Δ3 2等位基因突变率和流式细胞术检测 CCR5基因在细胞表面的表达 ,运用 One-wayANOVA( SPSS 1 0 .0 )进行统计学分析。结果　 70份不同民族标本中 ,发现 1例杂合子 (维吾尔族 ) ,其余均为野生型纯合子。傣族人群的 CD3 + CCR5 + 和 CD4+ CCR5 + 表达百分数明显高于其他 6个民族 ( P<0 .0 1 )。结论　 7个民族人群中 CCR5 -Δ3 2等位基因突变率很低 ,提示该人群对嗜巨噬细胞的 HIV-1感染的遗传易感性较强 ,尤以傣族人群更易感。更多还原

【Abstract】 Objective A mutant allele of the β chemokine receptor gene CCR5 bearing a 32 basepair(bp) deletion (denoted by CCR5 Δ32) which prevented cell invasion by the primary transmitting strains of HIV 1 has recently been characterized.To further characterize the risk of acquiring HIV,the HIV genetic diversity in a sub set of 6 minorities and Han populations were examined.Methods To get genotype and allele frequencies of CCR 5 and Δccr 5,PCRs were performed on genomic DNA samples.Surface expression of CCR 5 was measured by flow cytometry.One way ANOVA was used to determine statistical differences.Results Samples from 10 members of each minority were examined.There was a heterozygote in Weiwuer minority and no mutations were found among the other 69 samples investigated.The mean CCR5 expression of the Dai minority was significantly different from those of all other minorities both for CD3 +CCR5 + and CD4 +CCR5 +( P <0.01).Conclusion This pilot study shows the Dai minority may have a greater CCR 5 dependent susceptibility to HIV acquisition compared to the other minorities investigated.Because of this added risk factor,special prevention resources should be focused on this minority.Further studies of the biological risk in the Dai community are warranted.更多还原