【摘要】 目的:为研究瘢痕疙瘩Ⅰ、Ⅲ型胶原基因表达情况。方法:采用cDNA-mRNA原位杂交法观察正常皮肤、瘢痕疙瘩祛炎舒松-A(TA-A)治疗前及治疗后Ⅰ、Ⅲ型胶原基因表达情况,并进行Ⅰ型胶原天狼星红特异性染色,观察Ⅰ型胶原的分布和含量。结果:研究表明瘢痕疙瘩中Ⅰ、Ⅲ型胶原表达增加,经TA治疗后降低。结论:瘢痕疙瘩中胶原堆积是胶原基因表达增加所致,TA-A能抑制胶原基因表达,从而抑制胶原合成。更多还原

【Abstract】 Objective: To study the expression of types Ⅰ , Ⅲ collagen gene in keloid. In situ hybridization method was employed to measure the levels of pro αl( Ⅰ), and pro αl (Ⅲ ) mRNA in normal skin, and keloid tissue pro and post treatment with TA. In addition, a specific method of type Ⅰ collagen staining was used to measure the distribution and level. The results showed that type Ⅰ and Ⅲ collagen gene expression was increased in keloid. After treatment, the expression was decreased. The accumulation of collagen in keloid was the reason of increasing gene expression of type Ⅰ, Ⅲ collagen , TA - A can inhibit type Ⅰ ,Ⅲ collagen gene expression and the synthesis of collagen.更多还原