【摘要】 目的:研究塞来昔布对胃癌细胞株SGC-7901生长抑制及诱导凋亡的作用。方法:12.5～100μM的塞来昔布处理胃癌细胞株SGC-7901 24小时后,用MTT法测定塞来昔布对胃癌细胞的生长抑制率,流式细胞仪检测细胞周期、细胞凋亡率及MDR1表达水平。结果:12.5～100μM的塞来昔布对胃癌细胞均有抑制作用,首先表现为S期阻滞,继而出现细胞凋亡,且呈剂量依赖性。胃癌细胞MDR1表达水平在12.5μM药物浓度作用时MDR1表达上调,25～100μM药物浓度作用时MDR1表达水平明显下调,且随药物浓度的增高,MDR1表达水平逐渐下降。结论:塞来昔布通过诱导细胞凋亡对胃癌细胞具有明显抑制作用,同时塞来昔布具有下调胃癌细胞MDR1表达,逆转多药耐药性的作用,但在药物浓度较低时,则为上调MDR1表达。更多还原

【Abstract】 Objective:To study the effects of growth inhibition and apoptosis induced by celecoxib in human gastric cancer cells line SGC-7901. Methods;The gastric cell line was treated with 12. 5 - 100μM of celecoxib for 24 hours. MTT assay was used to determine the cell growth inhibition rate. Flow cytometry was used to examine cell cycle, cell apoptosis rate and MORI gene expression. Results:Celecoxib could inhibit the proliferation of gastric cells in vitro, which was associated with arresting of S phase and inducing apoptosis. The expression level of MDR1 gene decreased after treated with 25 - 100μM of celecoxib for 24 hours, but increased in 12. 5μM concentration. Conclusion:Celecoxib can induce apoptosis and block cell cycle of gastric cells. It also may reverse multi-drug resistance by descending the expression level of MDR1.更多还原