【摘要】 目的 :探讨在白血病细胞中细胞周期素zCyclin)G、CyclinD、P16、P2 1、P5 3等基因的蛋白表达水平和白血病细胞周期变化。方法 :建立白血病模型 (L615 ) ;采用免疫组织化学法检测小鼠的CyclinG、P5 3、P2 1、Cy clinD、P16和C fos、bcl 2的表达 ;流式细胞术检测小鼠白血病细胞的凋亡率和细胞周期。结果 :阳性组和复发组的CyclinG、CyclinD、C fos表达明显高于正常组和化疗组 ( P <0 .0 5 ) ,P16则相反 (P <0 .0 1) ;P2 1在四组中的表达差异无显著性意义 (P >0 .0 5 ) ;阳性组和复发组P5 3的表达为阴性 ,而化疗组与正常组表达差异无显著性意义 (P >0 .0 5 ) ;bcl 2四组表达差异无显著性意义 (P >0 .0 5 ) ;化疗组和阳性组的细胞凋亡率均较正常组增高(P <0 .0 5 ) ,且化疗组的凋亡率比阳性组的明显增高 (P <0 .0 1)。结论 :白血病的发生、发展可能与CyclinG、CyclinD、C fos基因的高表达 ,P16低表达有关更多还原

【Abstract】 Objective:To study expression of Cyclin G?P53?P21?Cyclin D?P16 and C-fos ?bcl-2 in leukemia mice. Method:By established mice models with leukemia (L615); the protein expression of Cyclin G?P53?P21?Cyclin D?P16 and C-fos ?bcl-2 were measured with immunohistochemical method;the rate of apototic cells and cell cycle were detected by flow cytometry.Result:Expression of Cyclin G?Cyclin D?C-fos in control group and relapse group was higher than that in normal group and chemotherapy group (P< 0.05) , expression of P16 was reverse(P< 0.01); expression of P21 showed no difference(P> 0.05), expression of P53 in control group and relapse group was negative , in normal group and chemotherapy group showed no difference(P< 0.05) , expression of bcl-2 showed no difference(P> 0.05) ; the rate of apoptotic leukemia cell in chemotherapy group and control group was higher than that in normal group(P< 0.05) , and chemotherapy group was obviously higher than normal group(P< 0.01).Conclusion:Leukemia is related to higher expression of Cyclin G?Cyclin D?C-fos gene and lower expression of P16, the rate of apoptosis of leukemia cell can be induced by chemotherapy.更多还原