【摘要】 目的:研究肝硬化患者血浆凝血—抗凝系统的变化,并探讨其临床意义。方法:肝硬化患者50例(代偿期30例,失代偿期20例),检测其血浆中凝血因子与抗凝蛋白。凝血因子抗原(F:Ag)用火箭电泳法,凝血因子活性(F:C)用一期凝血法或发色底物法;抗凝血酶原(AT:Ag)用免疫浊度法,抗凝血酶活性(AT:A)用发色底物法;蛋白C抗原(PC:Ag)用放射免疫法,蛋白C活性(PC:A)用发色底物法;补体1抑制剂(Cl-Inh:Ag与Cl-Inh:A)用免疫扩散法;组织因子途径抑制物抗原(TFPI:Ag)用ELISA法,组织因子途径抑制物活性(TFPI:A)用发色底物法。结果:肝硬化患者凝血因子除FⅧ外均有不同程度的降低,其中FⅤ与纤维蛋白原的降低见于失代偿期。肝硬化患者抗凝蛋白除TFPI外均降低。结论:由于凝血因子与抗凝因子多在肝脏制造,肝硬化时肝组织受到损伤致凝血因子与抗激因子制造障碍。其测定结果可用以评价肝脏损伤,防治出血或血栓形成,判断预后。更多还原

【Abstract】 Purpose: To study the changes for coagulative and anticoagnlative system of plasma in patients with hepatocinhosis and its clinioal impli-cation. Methods: 50 cases with hepatocirrhosis (30 cases compensation, 20 cases decompensation) was detected by different assay: Antigens of coagnlation factors are detected with rochet electrophoresis methods. Activity of coagulation factors is detected with one clotting stage way or colored substrate method. AT: Ag by inmune turbidimerry, AT: A by colored substrate rnethod. PC- Ag by radioimmunoassay, PC: A by colored substrate method. TPS:Ag and FPS: A by rocket immumoelectrpphoresis. C1-Jnh:Ag and C1-Inh: A by immunodiffusion technique.TFPI: Ag by ELISA assay,TFPI: A by colored substrate method. Results: The activity of F Ⅷ and eievated,the antigens and activity of other coagulation factors were decreased, but decreasing of F V and fib-rinogen only can be seen in decompensatory phase of cirrhosis. Anticoagulative protein levels of plasma in patients with hepatocirrhosis lower than control group except for TFPI. Conclusion: Due to aimost all coagutative factor and anticoagulative protein were made by liver decreased with certainly in palients with hepalocirrhosis. These markers are useful to evaluate liver daniage, predict bleeding or thrombosis,estimate prognosis.更多还原