【摘要】 目的 :研究屏氧酶 192谷氨酸 /精氨酸 (PON192 ,Gln/Arg)基因多态性与血浆脂质水平及心肌梗死(MI)的关系。方法 :应用多聚酶链反应 限制性内切酶片段长度多态性 (PCR RFLP)技术检测 132例MI与相同例数正常人PON 192基因多态性 ,血浆脂质水平测定按常规进行。结果 :发现PON 192基因型有三种 ,即纯合子突变型 (BB) ,杂合子突变型 (AB) ,正常型 (AA)。正常对照组中AA型频率 17.6 % ,AB型频率 4 5 .3% ,BB型频率37.1%。A等位基因频率为 4 5 .2 % ,B等位基因频率为 5 4 .8%。MI组AA型频率 10 .2 % ,AB型频率 4 1.3% ,BB型频率 4 8.5 %。A等位基因频率为 30 .7% ,B等位基因频率为 6 9.3%。两组对比等位基因频率差异有显著性意义。高密度脂蛋白水平在两组间差异无显著性意义。结论 :MI患者PON 192 ,B等位基因频率明显高于正常对照组 ,说明PON 192基因突变与MI的发生密切相关更多还原

【Abstract】 Objective:To study the relationship of polymorphisms of paraoxonase (PON192) gene with lipid levels and myocardial infarction (MI). Methods:The 132 MI patients and 132 normal subjects were recruited in the study .Their polymorphisms of PON192 gene were analyzed using PCR RFLP and their lipid levels were measured in our laboratory.Results:There were three kinds of genotype: AA(homozygous mutation ), AB(heterozygous mutaion) and BB (wild type). The frequencies of the three genotypes were as follows: AA(Gln/Gln) 17.6 %; AB (Gln/Arg) 45.3 %; BB(Arg/Arg) 37.1 %; A allele frequence was 45.2 % and B was 54.8 % in normal subjects and 10.2 %, 41.3 %, 48.5 %, 30.7 %, 69.3 % respectively in patients with MI. B allele frequence was higher in MI than in normal subjects. The plasmal levels of lipids were not differenct in two groups. Conclusion:There is a marked relationship the between polymorphisms of paraoxonase(PON192)gene with MI.更多还原