【摘要】 目的 :探讨E 选择素基因A5 6 1C多态性与 2型糖尿病 (T2 DM)并发冠心病 (CHD)的关系。方法 :用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)方法探查E 选择素基因A5 6 1C多态性在对照组、CHD组、T2 DM组、T2 DM并发CHD组中的基因频率分布。结果 :CHD组、T2 DM并发CHD组E 选择素的C等位基因频率明显高于对照组 (P <0 .0 5 ) ,不同基因型间血浆E 选择素水平差异有显著意义 (P <0 .0 1)。结论 :E 选择素基因A5 6 1C多态性与CHD及T2 DM并发CHD的发生有关更多还原

【Abstract】 Objective:To determine whether the E selectin gene A561C polymorphism is associated with type 2 diabetes mellitus(T 2DM), coronary heart disease(CHD) and diabetes complicated CHD.Methods:Genomic DNA was extracted from peripheral blood leukocytes. The allele frequency was investigated by means of polymerase chain reaction restriction fragment length polymorphism(PCR RFLP).Results:The frequencies of R allele in CHD and diabetic CHD group were significantly higher than that in normal control(P< 0.05 ) . Levels of plasma soluble E selectin were related to E selectin genotype.Conclusion:E selectin gene A561C polymorphism is associated with CHD and diabetic CHD.更多还原