【摘要】 目的　了解产超广谱 β 内酰胺酶 (ESBLs)的细菌分离、分布及对抗生素的敏感性。方法　用API或VITEK AMS系统 (Bio’Merieux ,法国 )鉴定菌种 ,用NCCLS推荐的初筛和确证法检测产ESBLs的细菌 ,并用K B法测定其对 14种抗生素的敏感性。结果　 2 0 0 0年武汉地区的2 69株大肠埃希菌、2 0 2株肺炎克雷伯菌、14 9株阴沟肠杆菌、67株费劳地枸椽酸杆菌、43株产酸克雷伯菌中ESBLs的检出率分别为 40 .9%、2 9.2 %、2 0 .1%、2 9.9%、和 2 3 .3 %。产ESBLs大肠埃希菌、肺炎克雷伯菌对亚胺培南全部敏感 ,产ESBLs的大肠埃希菌对阿米卡星的耐药率为 2 2 .4%,对其它抗生素有不同程度的耐药。结论　产ESBLs的菌株集中在肠杆菌科菌中 ,以大肠埃希菌、肺炎克雷伯菌、费劳地枸椽酸杆菌的ESBLs产出最高。阴沟肠杆菌、产酸克雷伯菌ESBLs的产出应引起重视。治疗其感染时应首选亚胺培南更多还原

【Abstract】 Objective To investigate the distribution and antibiotic susceptibility of producing extended spectrum beta-lactamases(ESBLs)bacteria.Methods API or VITEK AMS system were used to identify the bacteria;ESBLs were detected by NCCLS recommended methods and the susceptibilities to 14 kinds of antibiotics were tested by K B method.Results The incidences of ESBLs among 269 strains of Escherichia coli,202 strains of Klebsiella pneumoniae,149 strains of Enterobacter cloacae,67 strains of Citrobacter freundii and 43 strains of K.oxytoca found in Wuhan in 2000 were 40.9%,29.2%,20.1%,29.9%,7.7% and 23.3% respectively.All of the ESBLs producing E.coli and K.pneumoniae were susceptible to imipenem.The resistance rate of ESBLs producing E.coli to Amikacin was 22.4%,while these E.coli were resistant to other antibiotic at different levels.Conclusion The ESBLs producing bacteria mainly belong to Enterobacteriaceae E.coli,K.pneumoniae and C.freundii can produce higher level of ESBLs than others.The ESBLs produced by E.cloacae,K.oxytoca and E.cloacae are serious challenges for the control of infectious diseases.Imipenem is a good choice for the therapy of ESBLs producing bacteria.更多还原