【摘要】 目的　建立比色测定血清酸性α 醋酸萘酯酶总活性的方法。方法　用α 醋酸萘酯为底物 ,在 37℃、pH6 .2的酸性环境中经酶水解产生α 萘酚 ,然后与重氮试剂固蓝B偶联反应显色测定。结果　最适pH 6 .0～ 6 .4 ,底物浓度为 2mmol/L ,吸收峰波长为 5 2 0nm ,线性范围 0～ 2 5 0 0U/L ,酶促反应时间为 2 0min ,显色反应时间为 2 0min ,显色稳定时间为 2 0min ,批内、批间变异系数分别为 2 .6 7%和 5 .1 %。血红蛋白 1g/L和胆红素 1 72 μmol/L无干扰。枸橼酸钠、草酸盐、肝素、EDTA K2 常用浓度无抑制 ,氟化钠可抑制该酶活性。标本 2～ 8℃冰箱保存 7d结果无变化。正常参考值 (n =1 0 0 )为 (1 6 4 5± 378.5 )U/L( x± 1 .96s)。结论　该方法简便实用 ,适于各级实验室开展 ,可作为评价肝细胞变性坏死、肝纤维化、肝脏合成功能异常的一项新的血清学指标更多还原

【Abstract】 Objective To establish a colorimetric assay for detecting acid alpha naphthyl acetate esterase in serum.Methods When pH was 6.2 and temperature was 37℃,α naphthyl acetate was hydrolyzed by acid alpha naphthyl acetate esterase and it produced α naphthyl.Then colorimetric assay was performed with fast B salt diazotization reaction.Results The optimum pH was 6.0～6.4 and substrate concentration was 2mmol/L.Absorbent wavelength was at 520 nm.Linear range for enzyme determination was from 0 to 2 500 U/L.Time for enzymatic reaction was 20 minutes.Time for production of color reaction was 20 minutes.Time for color stabilization was 20 minutes.Intra assay CV value was 5.1% and inter assay CV value was 2.67%.When Hb concentration rose up to 1g/L and bilirubin to 172μmol/L,enzymatic activity was not interfered.When sodium citrate,sodium oxalate,heparin and EDTA K 2 was common concentration,enzymatic activity was not interfered,but NaF interfered for enzymatic activity.The results did not changed in seven days when sera were stored at 2～8℃ in refrigerator. The mean value in 100 healthy subjects was 1 645±378.5U/L( ±1.96 s ).Conclusions The method was simple and useful.It fit into every laboratory.It should be a new indicator for evaluation of hepatic function.更多还原