【摘要】 目的　建立一种丙型肝炎病毒型特异性PCR基因分型方法。方法　对 4 7份血液透析患者HCVRNA阳性的标本 ,用 5′非编码区 (5′ NCR) 1、2、3、1b型特异性引物 ,进行逆转录巢式PCR扩增分型。结果　 4 7份HCVRNA样本 4 3例可以分型 ;优势株为 1b亚型 ,1b及 1b相关型占总样本数的 87.2 3% (4 1 / 4 7) ;7例为混合感染 ,占可分型数的 1 6 .2 7% (7/ 4 3)。结论　本组血液透析患者感染的HCV基因型的分布可能与地区流行及医源性传播有关 ,其同源性有待核苷酸序列分析加以印证更多还原

【Abstract】 Objecyive A new PCR with the type specific primers designed according to the sequence of 5′non coding region (5′NCR) for genotyping HCV has been established.Methods Forty seven serum samples from heamodialysis patients were studied by this typing technique.Results Of forty three samples typed,fourty one were positive with the subtype 1b primers.Mixed infection was evident in seven samples in which there were bands associated with more than one genotype.The range of genotype may be related to the geographical areas or the multiple exposure in hospital.Conclusions The PCR products should be further identified by nucleotide sequencing to analyze their homology.更多还原