【摘要】 目的 :探讨有效提取膜迷路蛋白时十二烷基硫酸钠 (SDS)的用量。方法 :用二喹啉甲酸(BCA)法测定含不同浓度SDS的提取液所提取的豚鼠内耳膜迷路蛋白含量 ,并用SDS 聚丙烯酰胺凝胶电泳 (PAGE)分析其蛋白组分。结果 :0 .2 3%SDS、1%SDS、2 %SDS组所提取的蛋白量大于 0 .1%SDS组 ,同时电泳显示的条带更多 ,染色更深。 1%SDS组与 2 %SDS组效果又好于0 .2 3%SDS组 ,其中 2 %SDS组提取的量与 1%SDS组差别不大 ,但去垢剂 蛋白质之比明显升高。结论 :蛋白提取液中含 1%SDS ,去垢剂 蛋白质比为 5～ 6左右时对充分提取膜迷路蛋白较为合适。更多还原

【Abstract】 Objective:To determine the appropriate dosage of SDS when extracting membranous labyrinth proteins. Method:The yield of proteins extracted with various amounts of SDS were determined by BCA method, and the components of the extracted proteins were analyzed by SDS PAGE. Result:The amount of proteins extracted with 0.1 % SDS is less than that with 0.23 %,1% or 2% SDS, meanwhile it shows less bands than the other and the corresponding bands are stained weaker. 0.23 % SDS is less efficiently than 1% and 2% SDS in solubilizing proteins. There is no significant difference between 2% and 1% SDS in yield of proteins, while 2% SDS accompanies a higher ratio of detergent to protein. Conclusion:1% SDS in solutions is relatively suitable for extracting membranous labyrinth proteins extensively while the ratio of detergent to protein is from 5 to 6.更多还原