【摘要】 目的 :探讨白细胞介素 5 (IL 5 )及嗜酸性粒细胞阳离子蛋白 (ECP)在鼻息肉发病中的作用及相互关系。方法 :采用 pharmaciaCAP荧光免疫系统和ELISA双抗体夹心法对 30例鼻息肉患者 (鼻息肉组 )和 8例鼻中隔偏曲或阻塞性睡眠呼吸暂停综合征 (OSAS)患者 (对照组 )分别进行血清中ECP及组织匀浆中ECP、IL 5的检测。结果 :鼻息肉组匀浆中IL 5的水平明显高于对照组 ,其差异有显著性意义 (P <0 .0 5 ) ;鼻息肉组血清与匀浆中的ECP含量明显高于对照组 ,其差异亦有显著性意义 (P <0 .0 5 )。鼻息肉组匀浆中IL 5与血清中ECP水平呈明显正相关 (r=0 .5 98,P <0 .0 5 ) ;与匀浆中的ECP水平也呈正相关 (r =0 .4 5 1,P <0 .0 5 )。 结论 :ECP是嗜酸性粒细胞活化的标志 ,也是导致鼻腔炎症发生的重要因子 ;IL 5在鼻息肉组织中高表达 ,并与血清和组织中ECP水平密切相关 ,共同促进鼻腔炎症过程的不断加重。更多还原

【Abstract】 Objective:To evaluate the degree of recruitment and activation of eosinophils; to confirm the concentration and expression of IL-5 in nasal polyps; to study the correlation between eosinophilic activation and cytokine such as IL-5.Method: Blood and nasal specimens were collected from 30 patients with nasal polyps and 8 controls with deviation of nasal septum or OSAS. The serum ECP levels in two groups and the IL-5 and ECP in nasal polyp specimens or inferior turbinate mucosa were examined.Result:Concentration of IL-5 and ECP in nasal polyps tissue homogenates were significantly higher than those in inferior turbinate mucosas (P< 0.05). The same result was showed in serum ECP. The elevated IL-5 levels in polyp homogenates had a significant correlation with serum ECP(r= 0.598,P< 0.05) and polyp tissues ECP(r= 0.451,P< 0.05).Conclusion:The concentration of IL-5 increased in the nasal polyp tissues and the degree of expression showed a significant correlation with activated eosinophils.更多还原