【摘要】 目的 :观察分泌型天冬氨酸蛋白酶 (secretedasparticproteinase ,SAP)与念珠菌致病性的关系 .方法 :从住院病人中分离培养出 5 0株念珠菌 ,鉴定菌种后采用限制氮盐的培养基半定量测定天冬氨酸蛋白酶 ,并筛选蛋白酶阳性或阴性菌株 .经证实为系统感染的 10株菌株腹腔感染健康小鼠 .结果 :白色念珠菌天冬氨酸蛋白酶阳性率高达 90 % ,类星型和伪热带次之 .蛋白酶阳性或阴性的念珠菌对健康小鼠均无致病性 .结论 :天冬氨酸蛋白酶在正常条件下并不能增加白念菌的侵蚀性和致病力更多还原

【Abstract】 To investigate the correlation between candida albicans secreted aspartic proteinase and stain virulence. Methods: 50 strains of candida from patients in our hospital. The strains were identified by assimilation reaction. The enzyme was determined by semi-quantitative, which was on albumin agar plate Nitrogen-restricted media. The strains that can lead to systemic inflammation were applied to infect healthy kunming mice. Result: 90% candida albicans can produce this type of enyme. Enzyme-produced and non-enzyme-produced candida albicans were both not pathogenetic to this mouse. Conclusion: In general condition, aspartic proteinase can not increase the pathogenesis of candida albicans.更多还原