【摘要】 通过观察神经肽Y对人血管平滑肌细胞低密度脂蛋白受体表达的影响 ,以探讨神经体液因素在动脉粥样硬化发生中的重要作用。将体外培养的人血管平滑肌细胞分成对照组和不同浓度神经肽Y组 (浓度分别为 1 0 8mol L、1 0 7mol L、1 0 6 mol L和 1 0 5mol L) ,各组分别培养 6h、1 2h、2 4h和 4 8h ,然后经免疫荧光组织化学染色 ,在激光扫描共聚焦显微镜下定量检测神经肽Y对人血管平滑肌细胞的低密度脂蛋白受体表达的影响。结果发现 ,对照组低密度脂蛋白受体表达的平均荧光值在 2 4 2 8～ 2 5 2 7之间 ,且不同培养时间无显著性差异 (P >0 .0 5 )。而各神经肽Y组低密度脂蛋白受体表达的平均荧光值与对照组之间有显著性差异 (P <0 .0 5或P <0 .0 1 ) ,在不同的培养时间中 ,1 0 8mol L神经肽Y组平均荧光值在 1 798～ 2 4 0 8之间 ;1 0 7mol L神经肽Y组在 1 783～ 2 332之间 ;1 0 6 mol L神经肽Y组在 1 72 2～ 2 2 81之间 ;1 0 5mol L神经肽Y组在 1 5 90～ 2 0 1 0之间。与对照组相比 ,分别平均下降 1 5 .5 9%、1 9.78%、2 1 .91 %和 2 6 .83% ,且不同浓度神经肽Y组之间多有显著性差异 (P <0 .0 5 ) ,神经肽Y对成人血管平滑肌细胞的低密度脂蛋白受体表达的下调作用呈现一定的剂量和时间依赖效应。结果提示 ,更多还原

【Abstract】 Aim To detect the effect of neuropeptide Y (NPY) on the expression of low density lipoprotein receptors (LDLR) in human vascular smooth muscle cells (hVSMC). Methods The cultured hVSMC in vitro were divided into the control groups and the different concentration NPY groups (the concentrations of NPY were 10 8 mol/L, 10 7 mol/L, 10 6 mol/L and 10 5 mol/L). All groups were cultured for 6 h, 12 h, 24 h and 48 h respectively, then, were stained by the immunofluorescent histochemical staining and quantitatively assayed the effect of NPY on the expression of LDLR in hVSMC under laser scanning confocal microscope. Results The averaged fluorescent values of the LDLR expression in hVSMC of control groups were 2 428 to 2 527, and there were no significant differences among the different culture times (P>0.05), while there were significant differences between the averaged fluorescent values in NPY groups and those in control groups (P<0.05 or P<0.01). The averaged fluorescent values were 1 798 to 2 408 in 10 8 mol/L NPY groups, 1 783 to 2 332 in 10 7 mol/L NPY groups, 1 722 to 2 281 in 10 6 mol/L NPY groups and 1 590 to 2 010 in 10 5 mol/L NPY groups during the different culture times, decreasing averagely by 15.59%, 19.78%, 21.91% and 26.83% compared with control groups respectively, and there were significant differences among most of the different concentration NPY groups (P<0.01). NPY donwregulated the expression of LDLR in hVSMC with dose dependent and time dependent manner. Conclusion Neuropeptide Y could downregulate the expression of LDLR in hVSMC, then it probably resulted in blocking the LDLR mediated pathway, consequently LDL could accumulated in the vascular wall. This might be the important mechanism that neurofluid factor participated in the occurrence of atherosclerosis.更多还原