【摘要】 羧酸酯酶 (carboxylesterases)的过量产生是库蚊Culexpipiens对有机磷杀虫剂 (OP)产生抗性的主要机制。由est 3和est 2组成的酯酶超级基因座 (estersuper locus)的基因扩增是引起酯酶基因扩增的主要遗传学基础。通过淀粉电泳研究了采自广州、佛山、郑州的库蚊野生蚊虫种群 ,发现在这些种群中存在着扩增等位基因重组现象。该现象可能是蚊虫受到杀虫药剂的选择压力、等位基因多样性和等位基因型频率的影响。这将提供一个研究抗性进化的自然模型。更多还原

【Abstract】 Resistance to organophosphate (OP) insecticide in Culex pipiens is caused by over-produced nonspecific esterase. The ester super-locus is one of the main genome areas in this resistance, and presents multiple resistance alleles. We study three populations (Guangzhou, Foshan, Zhengzhou)of C. pipiens by starch gel electrophoresis, and find that there exist recombination phenomena between two amplified esterases allele in China. Through those data, we indicate that recombination phenomena is limited by selected pressure, the number of various amplified esterases allele, and the frequency of individual allele. This is the first report of recombination phenomena between two different amplified esterase alleles observed in field populations, and provides a good model to research resistant evolution.更多还原