【摘要】 目的　探讨T细胞淋巴瘤T细胞受体γ链基因重排的情况。方法　用免疫组化标记筛选 30例T细胞淋巴瘤、2例高度可疑为T细胞淋巴瘤和 10例淋巴结反应性增生 ,采用聚合酶链式反应扩增方法检测T细胞受体γ链基因重排。结果　 30例T细胞淋巴瘤中 2 7例和 2例高度可疑为T细胞淋巴瘤均出现T细胞受体γ链基因重排 ,10例淋巴结反应性增生均无T细胞受体γ链基因重排。结论　T细胞淋巴瘤病变中存在T细胞单克隆增生 ,支持肿瘤单克隆起源学说 ;T细胞受体γ链基因重排检测是区分T细胞淋巴瘤和淋巴结反应性增生的有效方法 ,且对T细胞淋巴瘤疑难病例的诊断有帮助。更多还原

【Abstract】 Objective To explore the rearrangement of T cell receptor gene γ chain(TCRγ) in T cell lymphoma.Methods Specimens from 30 cases of T cell lymphoma,2 cases that were difficult in diagnosis(malignant versus reactive T-cell infiltrate), and 10 cases of lymphaden reaction hyperplasia(RH). All above specimens were studied by immunohistochemical(IHC)methods and their TCRγ gene rearrangements were detected by polymerase chain reaction(PCR)technique.Results The positive TCRγ gene rearrangement were found in 27 out of 30 T cell lymphoma cases, two cases in difficulty diagnosis T cell lymphoma, and were not found in 10 RH patients.Conclusion The results provide an evidence to support the hypothesis of monoclonal origin of neoplasm. Detection of TCRγ gene rearrangement can be served as a genetic marker to differentiate T cell lymphoma from RH,and aid to the diagnosis of in difficult diagnostic cases.更多还原