【摘要】 目的 :探讨血管紧张素Ⅱ受体拮抗剂缬沙坦 (Valsartan)对实验性糖尿病大鼠肾皮质TGF - β1表达的影响 ,为糖尿病肾病的防治提供实验性理论基础。方法 :选择健康雄性SD大鼠 2 4只 ,任取其中 16只腹腔注射链脲佐菌素制成糖尿病大鼠模型。将糖尿病大鼠随机分为糖尿病缬沙坦治疗组 (A组 ,8只 ,缬沙坦 10mg·kg-1·d-1灌胃 ) ;糖尿病对照组 (B组 ,8只 ) ;其余 8只为正常对照组 (C组 )。分别于实验第 6周末测定各组大鼠血糖、血肌酐、尿白蛋白排泄率 ,对肾脏标本进行光镜观察 ,用图像分析仪测量各组大鼠平均肾小球面积、平均肾小球体积。并取各组大鼠肾皮质提取RNA ,用逆转录 -PCR(RT -PCR)方法对肾皮质TGF - β1mRNA表达进行半定量分析。结果 :在糖尿病第 6周末 ,B组上述指标较C组均有不同程度的升高 (P <0 .0 1) ,而A组则显著低于同时期的B组。其中 ,A组、C组尿白蛋白排泄率始终无统计学差异 ,肾小球平均面积、平均体积A组显著低于B组 (P <0 .0 1)。RT -PCR半定量结果分析显示 ,B组TGF - β1mRNA表达较A组、C组显著增高 (P <0 .0 1) ,A组TGF - β1mRNA表达较C组为高 (P <0 .0 1) ,但仍较B组为低 (P <0 .0 5 )。结论 :缬沙坦能够抑制肾组织TGF - β1mRNA的表达 ,减少糖尿病大鼠的尿白蛋白 ,减轻及延缓肾小球?更多还原

【Abstract】 Objective:To investigate the protectine mechanism of valsartan in the kidney of streptozotocin-induced diabetic rats.Methods:Sprague-dawley rats were randomly divided into diabetic model group treated with valsartan(A),diabetic model group (B) and normal control group (C).The plasma glucose,kidney mass/body mass ratio,serum creatinine,and 24 urinary albumin excretion rate were measured in the 6th week respectively.TGF-β 1 mRNA expression in renal cortinal was analysed with RT-PCR in the 6th week.And the kidney samples were observed with light microscope,mean glomerular transverse sectional area and mean glomerular volume were calculated by analysis system of the image.Results:After 6 weeks,kidney mass/body mass ratio,serum creatinine and 24 urinary albumin excretion rate in both diabetic group B were higher than that of normal group C( P <0.01),but they were decreased significantly in group A than that in group B.For the urinary albumin in two stages,there was no difference between A and C( P <0.05).Furthermore,the MGPA and MGV in group A were significantly smaller than that of group B ( P <0.01).The expression of TGF-β 1 mRNA in group B was significantly increased than that of group C ( P <0.01),at the same time the expression in group A was decreased than that of group B ( P <0.05). Conclusion: Valsartan could reduce the expression of TGF-β 1 mRNA, and the level of urinary albumin, and might prevent the glomerular sclerosis,and the pathological progress of kidney in diabetic rats.更多还原