【摘要】 对百部组织培养中影响不定根诱导的因素进行了研究。结果表明AgNO3 和IBA对生根诱导有较明显的影响 ,较优的浓度分别为 0 .5mg/L和 3 .0mg/L。随着继代培养代数的增加 ,生根率提高 ,第五代生根率达81 % ,比第一代提高 5 6%。继代培养基对生根诱导有较明显的影响 ,在IBA浓度为 0 .3mg/L的继代培养基下增殖的芽条 ,其生根率比IBA浓度为 0 .1mg/L和 0 .5mg/L的高 ,其根长亦比IBA浓度为 0 .1mg/L和 0 .5mg/L的长。在生根培养基中加入活性炭 ,对不定根的诱导起抑制作用 ,其生根率为 0。在生根培养基中加入碳素墨水可以提早百部生根时间 ,接种后第 1 0d即开始长根 ,且根的平均长度较长。愈伤组织对生根诱导有较明显的影响 ,基部带有愈伤组织的芽条的生根率明显高于基部没有愈伤组织的芽条。更多还原

【Abstract】 In order to apply tissue culture technique in rapid propagation of Stemona japonica (Bl) Miq., factors influencing root inducement of Stemona japonica (Bl) Miq. were studied. The results indicated that AgNO 3 and IBA had distinct effects on root induction. The best concentration of AgNO 3 was 0.1mg/L and that of IBA was 0.5mg/L. The rooting rates increased with generations of subcultures, which reach 81% in the fifth generation, higher by 56% compared with that in the first generation. The subculture media markedly affected root induction. The shoots derived from the multiplication media supplemented with 0.3 mg/L IBA had higher rooting rates and longer roots than those of 0.1 mg/L IBA and 0.5 mg/L IBA, respectively. The inhibitory effect on rooting was found in the media containing active charcoal and no rooting was observed during the culture in 35 days. However, when black ink was added into the rooting medium, the initiative time of rooting could be shortened, and rooting began at the 10th day after the inoculation. The average length of roots in the media added with black ink were longer than those of the control. The callus had obvious impact on root induction. The rooting rates of the shoots that had basal calli was obviously higher than those of the shoots without calli.更多还原