【摘要】 目的通过检测乳腺癌病人外周血和乳房肿块细针穿刺中肿瘤细胞的CEAmRNA拷贝数对乳腺癌的进行诊断并对血行转移进行监测。方法采用逆转录-实时定量PCR技术检测61例正常人外周血和58例乳房肿块病人(其中38例乳腺癌病人,15例良性小叶增生,5例乳腺纤维腺瘤)外周血、乳房肿块穿刺标本及肿瘤组织中的CEAmRNA拷贝数。结果61例正常人外周血中均无CEAmRNA的表达。乳腺癌病人的乳房肿块穿刺标本中CEA表达为89.5%,CEAmRNA拷贝数在106～107/ml;38例乳腺癌病人外周血中的表达阳性率为52.7%,浓度范围为102～104拷贝/ml;癌组织中CEA表达为100%,CEAmRNA拷贝数在104～107/mg;20例良性乳房肿块病人外周血及细针穿刺标本中均无CEAmRNA的表达。结论采用逆转录-实时定量PCR技术能特异、灵敏地诊断乳腺癌,并通过检测病人外周血中CEAmRNA来了解病人体内微转移的情况。更多还原

【Abstract】 Objective To diagnose breast cancer and monitor its metastasis from blood circulation by quantitative detection of carcinoembryonic antigen mRNA (CEA mR-NA)in the peripheral blood and the specimens from fine needle aspiration(FNA).Meth-ods CEA mRNA copy numbers were detected by reverse transcripatase polymerase chain reaction in the specimens of peripheral blood of61healthy people and the speci-mens of blood and FNA in58patiens with breast lumps.Results No CEA mRNA was detected in61healthy people and20patients with benign breast lesion.In38patients with breast carcinoma,CEA mRNA copy numbers were10 6 ～10 7 ml and expressional rate was89.5%in samples of FNA,and with10 2 ～10 4 /ml and52.7%in their peripheral blood,also10 4 ～10 7 /mg and100%in their cancer tissues respectively.Conclusions Quantitative detection of CEA mRNA by reverse transcripatase polymerase chain reaction can specially be used to diagnose breast carcinoma and monitor its metastatic status.更多还原