【摘要】 介绍了天然食品防腐剂乳链菌肽的生物合成及其相关基因的结构和功能。从乳链菌肽高产菌株Lactococcus lactis AL2的基因库中筛选到一个含有完整乳链菌肽生物合成基因簇的重组噬菌体λHJ-3,并在乳酸乳球菌中克隆和表达了编码乳链菌肽前体结构基因(nisZ)。更多还原

【Abstract】 The biosynthesis of nisin used as natural preservative for food and the structure and function of genes involved in biosynthesis is detailed. Regrouped bacteriophage λHJ-3 containing complete biosynthesized genes cluster of nisin is screened out from the gene files of Lactococcus lactis AL2 with high yield of nisin. The gene encoding the precursor of nisin is amplified by PCR using the λHJ-3 as the template and is cloned into pMG36e. The result of DNA sequence analysis indicates that NisinZ is produced by Lactococcus lactis AL2.更多还原