【摘要】 目的 :寻找肿瘤细胞特异性结合肽 ,为肿瘤诊断试剂及肿瘤药物的研制奠定基础。方法 :用培养的人源肿瘤细胞使裸鼠致瘤 ,将随机肽库从裸鼠的尾静脉注射到裸鼠体内 ,循环数分钟后取肿瘤组织及正常对照组织进行噬菌体筛选 ;经过 2～ 3轮体内筛选 ,获得在肿瘤组织中富集的肽段。结果与结论 :利用体内噬菌体展示技术 ,结合人源肿瘤细胞 （SMMC₇72 1和PLA₈0 1D）裸鼠致瘤模型 ,从随机十二肽库中成功地筛选到可以较高特异性地结合SMMC₇72 1肿瘤细胞或肿瘤血管的噬菌体多肽 ,通过对噬菌体单克隆进行测序 ,初步确定了几种可能的多肽基序（motif）。更多还原

【Abstract】 Objective:To select the peptides binding to human tumor cells specifically, and to lay a foundation for developing tumor diagnostic agents and antitumor drugs.Methods:Nude mice were inoculated with human tumor cells, then random phage_peptide libraries were injected intravenously （tail vein） into mice.After 2-4 min the mice were sacrificed and the phage rescued from tissues （tumor and control）.The phage recovered from the tissues was amplified and re_injected for two consecutive rounds.After selecting for 2-3 rounds in vivo , the phage_peptides that homed to the tumor tissues were obtained.Results and Conclusions: Some phage_peptides which could specifically bind tumor tissues or blood vessels were screened successfully from the random twelve_peptide pool by means of phage display in vivo combined with inoculating human_derived tumor cells （SMMC₇721 and PLA₈01D） to nude mice.After sequencing these positive phages′ ssDNA,several motifs were preliminarily determined.更多还原