【摘要】 目的 :从患者尸解肺组织中直接扩增冠状病毒部分聚合酶基因序列 ,为重症急性呼吸综合征 (非典型肺炎 )的病原确认提供依据。方法 :采用RT_PCR方法 ,从非典型肺炎病例标本中进行冠状病毒聚合酶基因的扩增与序列测定。采用ClustalX1.8软件进行系统发生树分析。结果与结论 :从非典型肺炎病例尸解肺组织、传代发病小鼠肺组织及分离物感染的细胞培养物中 ,可分别扩增出冠状病毒的DNA片段。测序结果显示其核苷酸序列与目前已知冠状病毒的同源性在 6 0 %左右 ,而与加拿大和美国最近报道的新型冠状病毒的序列是一致的更多还原

【Abstract】 Objective:To amplify and determine the sequence of partial polymerase gene of novel coronavirus isolated from lung tissue of severe acute respiratory syndrome(SARS)patients in mainland China, and to confirm SARS pathogen.Methods:DNA fragments of the polymerase gene were obtained by reverse transcription_polymerase chain reaction (RT_PCR) and the sequence homology and phylogenetic were analysed by the Neighbour Joining method of Clustal X 1.8.Results and Conclusions: The DNA fragments with expected length could be amplified by RT_PCR from the lung tissue of SARS patients and that of infected mouse and the cell cultures. The sequence homology analyses revealed that the sequence had about 60% homology compared with other known coronaviruses, and had 99% homology with novel coronaviruses isolated in USA and Canada.更多还原