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转PEPC基因水稻接种固氮菌的效应

Effect of Nitrogen-fixing Bacteria on PEPC Transgenic Rice ( Oryza sativa L. )

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【作者】 严建民闫春玲林敏张保明陈炳松焦德茂

【Author】 YAN Jian-min, VAN Chun-ling, LIN Min, ZHANG Bao-ming, CHEN Bing-song, JIAO De-mao( Institute of Application of Atomic Energy in Agriculture ,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. Institute of Application of Atomic Energy, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 3. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

【机构】 江苏省农业科学院原子能农业利用研究所中国农业科学院原子能利用研究所江苏省农业科学院农业生物遗传生理研究所 江苏 南京 210014北京 100094江苏 南京 210014江苏 南京 210014

【摘要】 利用15N-同位素技术研究转PEPC基因水稻及无PEPC基因水稻在接种不同固氮菌后的光合特性、产量性状和固氮能力的变化。结果表明:接种水稻联合固氮工程菌处理与未接种固氮菌处理相比,转PEPC基因水稻的PEPC活性、PS Ⅱ光化学效率(Fv/Fm)、净光合速率均明显提高,生物学产量和经济产量也相应增加了38.08%和26.86%;与无PEPC基因水稻相比,转PEPC基因水稻能明显提高水稻联合固氮工程菌的固氮效率。这种水稻光合与固氮相互促进的机理值得深入研究。

【Abstract】 The photosynthetic properties, yield character of PEPC transgenic rice inoculated nitrogen-fixing bacteria and nitrogen-fixing capacity of nitrogen-fixing bacteria inoculated in PEPC transgenic rice were studied by using l5 N-isotope tracer technique. The results showed that the PEPC activity, PS Ⅱ photochemical efficiency and net photosynthetic rate of PEPC transgenic rice inoculated nitrogen-fixing bacteria increased significantly under high light intensity . The dry matter of plant and grain yield of PEPC transgenic rice increased 38.08% and 26.86% under it was inoculated with nitrogen-fixing bacteria. Compared with no PEPC gene rice, the PEPC transgenic rice could enhance nitrogen-fixing capacity of nitrogen-fixing bacteria. The mechanism of acceleration between photosynthesis of PEPC transgenic rice and nitrogen fixation of nitrogen-fixing bacteria in PEPC transgenic rice need to be studied deep.

【关键词】 转PEPC基因水稻固氮菌
【Key words】 PEPC transgenic ricenitrogen-fixing bacteria
【基金】 国家973项目“杂交稻高光效生理育种途径”(J1998010100)
  • 【文献出处】 江苏农业学报 ,Jiangsu Journal of Agricultural Sciences , 编辑部邮箱 ,2003年02期
  • 【分类号】S511
  • 【被引频次】6
  • 【下载频次】118
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